Development and Validation of a UPLC Method for the Determination of Albendazole Residues on Pharmaceutical Manufacturing Equipment Surfaces

In Pharmaceutical industries, it is very impo remove drug residues from the equipment and areas used. The cleaning procedure must be validated, so special attention must be devoted to the methods used for analysis of trace amounts of drugs. A rapid, sensitive and specific reverse phase ultra chromatographic (UPLC) method was developed for the quantitative determination of Albendazole in cleaning validation swab samples. The method was validated using an ACQUITY HSS C 2.1mm, 1.8μ column with a isocratic mobile phase c mixture of 1.36g of Potassium dihydrogenphosphate in 1000mL MilliQ water, 2mL of triethylamine and pH adjusted to 2.3 ± 0.05 with ortho-phosphoric acid, Acetonitrile and Methanol (50:40:10 v/v). The flow rate of the mobile phase was 0.5 mL min column temperature of 35C and detection wavelength at 254nm using PDA detector. The injection volume was 2μl. Cotton swabs, moisten with acetonitrile were used to remove any residue of drug from stainless steel, teflon, rubber and silicon plates production equipment surface and the mean extraction found to be 91.8. The selected chromatographic condition was found to effectively elute Albendazole with retention time of 0.67 proposed method was found to be linear over the range of 0.2 to 150μg/mL and correlation coefficient obtained is proposed method was found to be accurate, precise, reproducible and specific and it can also be used for routine quality control analysis of these drugs in biological samples either alone or in combined pharmaceutical dosage forms. Keywords—Cleaning validation, Albendazole, residues, swab analysis, UPLC.